A simple non- invasive technique which was developed on the basis of ancient Egyptian knowledge called as Punyakoti test (Veena, 1997) was used to detect early pregnancy at day 26 post insemination. The present study was carried out to evaluate the accuracy of Punyakoti test, a seed germination inhibition test for early pregnancy diagnosis in graded Murrah buff aloes. The urine samples were collected from 20 buff aloes early in the morning on day 26 post insemination and were subjected to pregnancy diagnosis using this test. Urine was diluted with distilled water in sterile petri dish in the ratio of 1:14 and 15 good quality wheat seeds were added to each petri dish. A signifi cant inhibition of seed germination a er 48hrs and shoot length a er 5 days was taken as criteria for declaring true pregnancy and confi rmation of pregnancy was done by rectal palpation on days 45-60 post insemination. A significant difference (P<0.05) was noticed between pregnant

and non-pregnant buffaloes regarding germination inhibition percentage and shoot length (cm) which was recorded as 70.66 ±1.63; 3.33 ± 0.074 and 45.99 ± 1.84 ; 5.44 ± 0.17, respectively. This test was 66.66 per cent accurate in diagnosing pregnancy but 90.90 per cent accurate in diagnosing non-pregnant animals.